Regulation of the Nucleosome Repeat Length In Vivo by the DNA Sequence, Protein Concentrations and Long-Range Interactions
Figure 4
NRL dependence on the concentration of linker histones.
A) Experimentally determined NRL as a function of the concentration of histone hH1.4 [33]. B) Theoretically predicted NRL as a function of H1 activity. KH1 – binding constant; [H1] – concentration of free H1 in solution; m(H1) = 15 bp. C) A scheme illustrating the refined model for nucleosome-H1 arrangement: different configurations of bound H1 around nucleosome are allowed, but not more than a critical number of H1 per nucleosome. D) Dots - experimental NRL data from Woodcock et al. [34] for different mouse cell types. Solid line - theoretical prediction. m(H1) = 15 bp, KH1* c0(H1) = 0.0035, w(0, NCP, NCP) = 11.