Dynamics of SIN Asymmetry Establishment
Figure 4
Sensitivity of asymmetry establishment timing on Cdc11 modification efficiencies.
Efficiencies of SIN and Cdk phosphorylation (A) and ppC and ppS dephosphorylation (B) on the time it takes to reach asymmetry in SIN activity (inflection point in Byr4Old curve). Small decrease in SIN efficiency on Cdc11 phosphorylation advances asymmetry (this is what was observed for the cdc11-S5A mutant, noted with a green arrow), while major decrease in this efficiency delays the transitions and eventually leads to high Byr4 (∼multinucleate) phenotype. Increase in this efficiency leads to SIN hyperactivation (∼multisetpate) phenotype. Decrease in Cdk efficiency has no major effect on asymmetry, but increase in this delays the transition and can lead to SIN hyperactivation. Increase in ppC seems to have no effect on asymmetry timing, while increase in ppS can lead to Byr4 hyperactivation. All wild type parameter values are normalized to 1, thus horizontal dotted lines show the wild type timing of asymmetry establishment.