New Universal Rules of Eukaryotic Translation Initiation Fidelity
Figure 6
A–C. Genomic profiles of number of ATGs in the first thirty codons prior to, and following the START ATG, in comparison to 20 randomized genomes that maintain the amino acid order of each gene, the genomic GC content and codon usage bias, in the three frames (each sub-figure is related to one frame, A. Frame 0, B. Frame 1, C. Frame 2) for 33 eukaryotes (each row is one organism; each position within a row is related to a specific coordinate of the ATG codon before (5′UTR) or after the beginning of the ORF; Methods).
Left part: There are three possible colors: Red – denotes cases where in that position the random genome includes significantly more ATGs than the real genome; green – denotes cases in which in that position the random genome includes significantly less ATGs than the real one; black – denotes that the random and real genomes are not significantly different in that position. Right part: the regions (5′UTR and ORF) of significantly lower number of ATG codons relatively to the randomized genomes for each organism and each frame (Methods). In all frames, the number of ATG codons in the 5′UTRs is significantly lower than expected. In frame 1 and 2 (according to our randomization frame 0 is identical in the random and real genome) the number of ATGs in most of the organisms is significantly lower in the first five codons. See Supplementary Figure S34 for permuted randomization (Methods).