Testing Biochemistry Revisited: How In Vivo Metabolism Can Be Understood from In Vitro Enzyme Kinetics
Figure 1
The glycolytic and fermentative pathway as they were modeled in this study.
Metabolites are depicted in bold face, allosteric regulators in regular, enzymes in italics and branching pathways underlined. GLCo: extracellular glucose, GLCi: intracellular glucose, G6P: glucose 6-phosphate, F6P: fructose 6-phosphate, F16BP: fructose 1,6-bisphosphate, DHAP: dihydroxyacetone phosphate, GAP: glyceraldehyde 3-phosphate, BPG: 1,3-bisphosphoglycerate, 3PG: 3-phosphoglycerate, 2PG: 2-phosphoglycerate, PEP: phosphoenolpyruvate, PYR: pyruvate, ACE: acetaldehyde, EtOH: ethanol, HXT: hexose transport, HXK: hexokinase (EC 2.7.1.1), PGI: phosphoglucose isomerase (EC 5.3.1.9), PFK: phosphofructokinase (EC 2.7.1.11), ALD: aldolase (EC 4.1.2.13), TPI: triose-phosphate isomerase (EC 5.3.1.1), GAPDH: glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), PGK: 3-phosphoglycerate kinase (EC 2.7.2.3), GPM: phosphoglycerate mutase (EC 5.4.2.1), ENO: enolase (EC 4.2.1.11), PYK: pyruvate kinase (EC 2.7.1.40), PDC: pyruvate decarboxylase (EC 4.1.1.1), ADH: alcohol dehydrogenase (EC 1.1.1.1).