Genome-Wide Association between Branch Point Properties and Alternative Splicing
Figure 6
Predicted human branch points.
A – Histogram representing the distribution of BS positions relative to the AGEZ-defining AG-dinucleotide (a3 in Figure 1). Grey region represents positions that are biased by the presence of the AG dinucleotide. The dashed red line represents the leftmost point where the distribution is different from an expected uniform distribution. The AG dinucleotide exact position is shown on the x-axis. For this plot, top scoring candidates over the last 500nt were considered in order to obtain the left background tail. For visualization purposes only positions from −30 to +30 nts relative to the AG are shown. B – Pie chart showing the number of introns in the initial dataset (N = 183187) for which no predictions were obtained (None), no predictions falling inside the 1st AGEZ were obtained (None in AGEZ), the top prediction inside 1st AGEZ has a negative SVM score (Negative scoring) and the top prediction inside the 1st AGEZ scores positively (Positive scoring). C – Histogram showing the distribution of predicted BS distances relative to the 3SS. Only top scoring candidates inside the AGEZ were considered.