Endothelial Cell Capture of Heparin-Binding Growth Factors under Flow
Figure 9
Simulations show reduced binding with increased flow rate.
(A) Simulations for control (•), and HSPG-deficient cells (○), were run modeling injection of FGF-2 (1 ng) into the system and run at varied flow rate. 30% non-specific loss of FGF-2 in the reservoir was incorporated. (B) Cell-bound+internalized FGF-2 as a function of injection concentration at 5 min as a function of flow rate is shown. Simulations performed at 0.63 (•), 1.8 (○), and 3.0 (▪) mL/min pulsatile flow(1.26, 3.6, and 6 mm/sec, respectively). Each cell on the cell-lined hollow fiber expressed 1×104 FGFR/cell and 2.5×105 HSPG/cell.