Computational Model of Membrane Fission Catalyzed by ESCRT-III
Figure 8
Imaging of the ESCRT-III (CHMP2A-CHMP3) assembly.
(A) Electron micrograph showing an ESCRT-III tubule terminating into a hemispherical end-cap structure. (B) The closed end of the tubule after image processing. (C) Extracted edge of the ESCRT-III end-cap. (D) Fit of the extracted edge into the image. (E) Fit of a circle into the edge profile. The methods and experimental details for (A)–(E) are presented in [34]. (F) Cross sections of CHMP2A-CHMP3 end-capped tubular structures observed by cryo-electron tomography (see Materials and Methods). The images reveal the CHMP2A-CHMP3 protein layer and also the position of MBP proteins fused to the N-terminus of CHMP2A. The scale bar corresponds to a distance of 40nm.