mTORC1 in Thymic Epithelial Cells Is Critical for Thymopoiesis, T-Cell Generation, and Temporal Control of γδT17 Development and TCRγ/δ Recombination
Fig 7
Severe iNKT cell developmental defect in Rptf/f-Foxn1Cre mice.
Thymocytes and splenocytes from 10-d- or 6-wk-old Rptf/f (WT) and Rptf/f-Foxn1Cre (KO) mice were stained with PBS57 loaded CD1d-Tetramer (CD1dTET), TCRβ, and other indicated molecules. a. CD1dTET and TCRβ staining of live-gated thymocytes. The gating strategy is shown in S8A Fig. b. Percentages of iNKT cells. Each circle and square represent one Rptf/f and Rptf/f-Foxn1Cre mice, respectively (10 d, n = 3; 6 wk, n = 4). Horizontal bars represent means and SEM. c. Absolute numbers of iNKT cells. Each circle and square represent one Rptf/f and Rptf/f-Foxn1Cre mice, respectively (10 d, n = 3; 6 wk, n = 4). Horizontal bars represent means and SEM. d. iNKT cells to cαβT cell ratios in individual mice (10 d, n = 3; 6 wk, n = 4). e. Representative dot plots show CD44 and NK1.1 expression in gated iNKT cells. The gating strategy is shown in S8B Fig. f. Percentages of indicated iNKT cell populations (n = 4). g. Absolute numbers of indicated iNKT cell populations (n = 4). Data shown are representative of or are calculated from at least three experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001 determined by tailed Student’s t test.