PROTEIN TARGETING TO STARCH Is Required for Localising GRANULE-BOUND STARCH SYNTHASE to Starch Granules and for Normal Amylose Synthesis in Arabidopsis
Fig 9
Expression of GBSS-GFP in Arabidopsis ptst mutants.
Soluble (S) and insoluble (I) protein fractions of leaf extracts were subject to immunoblot analysis with GFP antibodies. Three independent lines generated in both wild-type and ptst-1 backgrounds are shown, each with varying expression levels. Starch-bound proteins are contained in the insoluble fraction. The small difference in migration between soluble and insoluble fractions was reproducibly observed and may be due to differences in sample preparation and composition.