Carbonic Anhydrase Generates CO2 and H+ That Drive Spider Silk Formation Via Opposite Effects on the Terminal Domains
Figure 2
CA activity and Azure blue staining of histological sections from (A) the sac of a Tegenaria sp. major ampullate gland, (B) E. australis minor ampullate gland, (C) A. diadematus aggregate gland duct, (D) Tegenaria sp. tubuliform gland, and (E) the third limb of the duct of an A. diadematus major ampullate gland. Black precipitates represent CA activity (arrow heads). In (A) the glandular lumen is labeled and the dotted arrow points towards the duct. Nuclei are indicated by (N) and the lumen by (Lu). Scale bar, (A) 50 µm and (B–E) 20 µm.