Dual PDF Signaling Pathways Reset Clocks Via TIMELESS and Acutely Excite Target Neurons to Control Circadian Behavior
Figure 10
PDF induces depolarization and increase in firing frequency in DN1p neurons.
(A) Representative current clamp recordings obtained from UAS-CD8 GFP;Clk4.1M-Gal4 male flies showing the depolarization and increase in firing frequency in control or in PDFR mutant (WT in black, PDFR− in red, n = 3). (B) Representative recordings showing the membrane potential in the presence of TTX in control (black, Δ = 9.2±0.6 mV, n = 3) or with a PKA inhibitor (H89- blue, Δ = 7.8±0.5 mV, n = 3). Mean ± SEM are shown in the histogram (C). (D) Representative recordings showing the PDF induced depolarization control (black, [Δ = 11±1.6 mV, and Δ = 3.4±0.46 Hz n = 5]) or in PKA-R1dn (blue, [Δ = 10.7±1.73 mV, and Δ = 4.7±0.64 Hz n = 4]). Mean ± SEM are shown in the histogram (E).