Three-Dimensional Reconstruction of Bacteria with a Complex Endomembrane System
Figure 1
3D reconstruction of bacteria with a complex endomembrane system.
(A) One slice of the tomogram is represented with the different structural features modeled. The OM is in green, the IM in cyan, the DNA in yellow, OM invaginations are in pink, and the Poly-P granule in dark blue. Scale bar is 500 nm. (B) Schematic of the cellular organization of G. obscuriglobus, not to scale. The periplasmic and cytoplasmic spaces are in dark and pale grey, respectively. Other colors are the same as in (A). (C and E) The modeled volume of one cell, sliced roughly through the middle, is represented in different orientations. Two views through the full volume are represented. (D) A slice through the same orientation as in panel (C) might give the false impression of a compartment surrounding the DNA. (F) Full volume representation without the membranes showing the five fragments of DNA, the crateriform structures, and the granule.