Lineage Analysis of Drosophila Lateral Antennal Lobe Neurons Reveals Notch-Dependent Binary Temporal Fate Decisions
Figure 8
The temporal transition from Chinmo to Br-C is not affected by loss of Notch signaling.
(A) The asense-GAL4-labeled wild-type, spdo, and Su(H) lAL clones (white) immunostained for Chinmo (magenta) and Br-C (blue). Single confocal planes at superficial, middle, and deep layers are shown. The white signals indicated by arrows are background clones. Scale bar: 10 µm. (B) The illustration shows the composition of the lAL lineage at 70 h ALH based on the Chinmo and Br-C antibody staining. At the superficial layer, the NB, GMCs, and newly generated neurons are negative for both Chinmo and Br-C. At the middle layer, the young neurons are positive for Br-C but negative for Chinmo. The neurons located slightly deeper are positive for Chinmo and Br-C, and the earlier born neurons in the deep layer are only positive for Chinmo. (C) The table shows the numbers of cells positive for Chinmo, Br-C, or both in asense-GAL4-labeled wild-type (WT), spdo, and Su(H) lAL clones at 70 h ALH.