The Circadian Neuropeptide PDF Signals Preferentially through a Specific Adenylate Cyclase Isoform AC3 in M Pacemakers of Drosophila
Figure 1
Data collection of FRET responses and transgenic RNAi screen of ACs potentially coupled to PDF receptor in M cell pacemakers.
(A) Raw FRET imaging data (CY/CC) collected for 10 min (each trace represents an individual cell recorded as an ROI) to show FRET loss in response to a bolus of PDF (marked by arrow) and recoveries to baseline. (B) The scatter plot represents the data shown in 1A and retains its color-coding. For each trace, the maximal deflection from its value at the initial time point is computed as “percent FRET loss” and represented as a single point. Error bars represent SEM. (C) Double-stranded RNAi directed against 11/12 genes known to encode known adenylate cyclases in the Drosophila genome. All genotypes include Pdf-gal4;Epac1camps and one copy of UASRNAi (except for control). Error bars denote SEM. *** p<0.001, ** p<0.01 (compared with control).