Combinatorial Activation and Repression by Seven Transcription Factors Specify Drosophila Odorant Receptor Expression
Figure 1
An RNAi screen identifies seven TFs required for OR expression.
(A) Whole mount preparations of antenna from the two screening rounds (GFP in black). In the first round, expression of Or98a-CD8::GFP and Or23a-CD8::GFP in two mid-antennal domains (light blue and orange oval) were analyzed. In the second round, Or92a-CD8::GFP expression in the most proximal (dark blue oval) antenna domain and Or47b-CD8::GFP expression in the most distal (red oval) antenna domain were analyzed. (B) Statistics from the screen is depicted as a graph, summarizing the number of IR lines that did not affect OR expression (Wt, white), led to lethality (Lethal, grey) or lost OR expression (Loss of OR expression, Green). (C) Phenotype summary for the seven TF-IRs and the analyzed OSN classes, wild-type OR expression (grey dots) and loss of OR expression (black dots). (D) Antenna from each TF-IR with representative OR expression phenotypes. (E) Whole mount antennal lobe with the Or92a-CD8::GFP OSN projections shown in green and the synaptic marker, nc82, delineating the glomeruli of the antennal lobe, in magenta. The boxed region indicates the antennal lobe area in the right panel, which compares the RNAi and mutant phenotypes of acj6, sim, xbp1, zf30c. Note the loss of Or92a in both the mutant and RNAi lines.