Functional Dissection of the Proton Pumping Modules of Mitochondrial Complex I
Figure 3
LILBID mass spectra of purified subcomplex nb8mΔ.
The predominant series of LILBID-peaks from the subcomplex indicated a protein mass of ∼645 kDa (red ticks). A series of minor peaks (green ticks) indicate that the rather loosely attached subunit ST1 was dissociated during ionisation from the major fraction even under ultrasoft conditions.