Gene Gain and Loss during Evolution of Obligate Parasitism in the White Rust Pathogen of Arabidopsis thaliana
Figure 7
P. capsici test for delivery motif.
To identify known and new classes of transfer motifs in A. laibachii, the P. capsici–N. benthamiana translocation assay was used. This test system is based on Avr3a-mediated avirulence in plants carrying R3a [68]. (A) virulence assay to show that transgenic P. capsici is not impaired in growth; (B) Hypersensitive response (HR) assay on R3a-carrying plants for delivery assay. The Avr3a RxLR translocation domain is replaced by the N-terminus of different A. laibachii effector candidates. For the assay, RXLR1, CRN3, and CHXC9 carrying the newly identified CHXC motif were used. Our results validate that known motifs like the CRN motif are functional while the selected RXLR shows low delivery efficiency. CHXC9 shows the same efficiency as Avr3a does, and dependency of the CHXC motif could be identified (statistical analyses using the Tukey test; means with the same letter are not significantly different; error bars denote standard error of the mean). wt, wild type.