Subtle Alterations in PCNA-Partner Interactions Severely Impair DNA Replication and Repair
Figure 5
In vivo phenotypic analysis of PCNA mutants.
(A) Non-viable strains containing PCNA mutants with increased binding affinity for Rad27 as the sole version of PCNA. Yeast strains containing WT PCNA expressed from a URA3 plasmid and mutant PCNA genes expressed from a LEU2 plasmid are viable (middle). Upon selection for loss of WT PCNA using 5-floororotic acid (5FOA), PCNA mutant strains are non-viable (right). (B) MMS (middle) and HU (right) sensitivity of strains containing PCNA mutants with increased binding affinity for Rad27, Pol32, Rad30, Msh6, and Ung1 and strains containing WT PCNA in which the same partners were deleted. For binding characterization and sequences of the mutants, see Table 1.