Involvement of Lgl and Mahjong/VprBP in Cell Competition
Figure 2
Loss of Mahj function induces cell competition in Drosophila wing disc epithelium.
(A) Wing and leg discs of homozygous mahj1 mutant third-instar Drosophila larvae stained with anti-Ci155 (red) and anti-Engrailed (green) antibodies. (B–F) Wing discs with mahj−/− (lacking GFP), wild-type (expressing GFP strongly), and mahj+/− clones (expressing GFP moderately) at 72 h (B), 96 h (C, E, and F), and 120 h (D) after clone induction. (E) A Z-stack projection of confocal images of a wing disc. Basally extruded apoptotic cells were excluded from the analysis. (F) Magnified images of (E). (E and F) Apoptotic cells were labeled with anti-cleaved Caspase-3 antibody (red). Nuclei were stained with DAPI (blue). (G) Quantification of apoptotic cells in the mahj1 mosaic wing discs showing non-cell-autonomous apoptosis in mahj−/− cells. The results represent means±SD (n = 34 discs, *p<0.001).