Cadherin-2 Controls Directional Chain Migration of Cerebellar Granule Neurons
Figure 6
Migratory pac−/−R cerebellar GCs show reduced contact stability and adherens junctions.
(A–D) Comparison of contact duration between individual gata1:GFP GCs in time-lapse sequences (white arrowheads) reveals that lack of Cadherin-2 in pac−/−R GCs significantly reduces the duration of stable cell-cell contacts (E, WT: 95.3±10.9 min versus pac−/−R: 36.8±5.6 min, n = 29). (F–H) TEM analysis reveals that AJs between pac−/−R cerebellar neurons are largely absent (G), while numerous AJs are found between individual WT cerebellar neurons (F). (H) pac−/−R cerebellar neurons appear disorganized (compare with Figure 1J). (I) Quantification of TEM images (3.8±0.9 AJs, n = 10, in WT versus 0.3±0.2 AJs, n = 7, in pac−/−R-cerebella).