Omega-3 Fatty Acids and Inflammation: Novel Interactions Reveal a New Step in Neutrophil Recruitment
Figure 8
The effect of adhesion on the expression of l-selectin and CD11b on neutrophils.
Neutrophils that were freshly isolated, migrated across endothelial cells stimulated with TNF or adherent to the surface of EPA-supplemented endothelial cells (EC) activated with TNF were harvested and the expression of (A) CD11b or (B) l-selectin (CD62L) assessed by flow cytometry. Data are mean±SEM of four experiments; ANOVA showed significant effect of treatments in (A) (p<0.01) and (B) (p<0.05). In (A and B), Dunnett's test showed significant difference between −EPA and freshly isolated cells (*p<0.01 and *p<0.05, respectively), but not between +EPA and freshly isolated cells.