Assembly of the Murine Leukemia Virus Is Directed towards Sites of Cell–Cell Contact
Figure 4
Assembly followed by transmission of MLV from virus-producing cells to target cells.
(A) Selective frames from Video S2 monitoring the assembly of MLV particles (Gag-YFP, red) in HEK293 cells expressing dynamin2-CFP (green) followed by the transmission to target XC cells expressing receptor mCAT1-CFP (green). Six representative particles were labeled A–F. The last panel represents single-particle tracking analysis for these particles. The size bar corresponds to 10 µm. (B) Display of the YZ movement of particle E shown in (A). (C) Single-particle tracking analysis of mCAT1/dynamin2-CFP (green), Gag-YFP (red), and particle motility (blue) from producer to target cell of particle E over time. (D) Analysis as in (C) for particles A–F.