Aneuploidy and Improved Growth Are Coincident but Not Causal in a Yeast Cancer Model
Figure 2
The mcm4Chaos3/Chaos3 mutant generates a subpopulation of genetically unstable cells.
(A) The mutant produces heterogeneous offspring. (i) Wild-type cells produce uniform sized colonies. (ii) The mutant produces heterogeneous sized colonies. The arrowheads point at representative minute colonies, S1, S2, and S3. Long black arrows indicate the lineage of colonies that were analyzed. L1 and S1 are a large and a minute colony derived from a streak of a large colony of the mutant. (iii) and (iv) are colonies derived from S1 and L1, respectively. (iii) Heterogeneous colony morphologies include giant (G1-1, G1-2), serrated (white arrow), and minute (S1P) colonies. The inset is a magnification of the heterogeneous colonies. (v) S1P gives rise to heterogeneous colonies including giant colonies such as G1P. (vi) Large colonies (L1P) consistently give rise to both large and minute colonies. Scale bar of 1 cm is shown. (B) Histograms of colony size of wild type (normal distribution) and mcm4Chaos3/Chaos3 (bimodal distribution). (C) The lineage of strains presented in Figures 2 and 3. Ancestral progenitor is represented by a black circle. Different minute colonies are color coded. Giant colonies derived from the same ancestral minute colony are coded with the same color. The number under each strain is the DT (h). S, minute; L, large; G, giant; P, progeny.