Self-Organization of Dynein Motors Generates Meiotic Nuclear Oscillations
Figure 1
SPB Movement is Driven by Pulling Via the Leading Microtubules
(A–D) SPB oscillations in cells expressing Sid4-GFP (SPB marker, indicated by asterisks) and GFP-alpha2-tubulin. Each panel shows selected images from a time-lapse sequence of a cell, together with a scheme of the SPB and microtubules at the time of laser ablation. The red bolt indicates the position of ablation; the black arrows indicate the direction of SPB movement. (SPB position as a function of time for Figure 1B–1D are shown in Figure 6D–6F). (A) Control cell without laser ablation.
(B) Laser ablation of the leading microtubules induced a change of direction of the SPB movement (n = 19 out of 21 cells; in the remaining two cells another microtubule pulled the SPB in the same direction).
Laser ablation of the (C) trailing microtubules (n = 19/19), or (D) the tip of the leading microtubules (n = 10/10) did not affect the SPB movement. Scale bars, 2 μm.