Cdk5 Regulates Accurate Maturation of Newborn Granule Cells in the Adult Hippocampus
Figure 7
Synaptic Integration of Cdk5-Deficient Newborn Granule Cells Extending Aberrant Dendrites
(A) Confocal microscopy revealed that aberrant dendrites extending from DNcdk5-expressing granule cells (arrows in [A]) traveling along the GCL (NeuN, red) were covered with spiny processes.
(B and C) Serial section electron microscopy showed synapse formation as defined by the presence of the following on at least one section: a clear postsynaptic density, at least four presynaptic vesicles within 100 nm of the presynaptic membrane, and a well-defined synaptic cleft. Note the postsynaptic density of the GFP-labeled, electron-dense dendritic spine and opposing vesicles in the axon terminal. A three-dimensional reconstruction of the synapse shown in (B) is depicted in (C).
The scale bar in (A) represents 10 μm, and the scale bars in (B and C) represent 0.5 μm. HL, hilus.