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High-Throughput In Vivo Analysis of Gene Expression in Caenorhabditis elegans

Figure 1

Analysis Pipeline for GFP Expressing Strains

Strains with nonubiquitous embryonic expression prior to 2-fold stage were stabilized for 4-D analysis, and all postembryonic strains were briefly assessed for their expression pattern complexity and then assigned to either the confocal microscope or the stereomicroscope for detailed observations. Once the expression analysis was complete, the data were sent to the public domain, and the strains were sent to the CGC (Caenorhabditis Genetics Center).

Figure 1

doi: https://doi.org/10.1371/journal.pbio.0050237.g001