Rb-Mediated Neuronal Differentiation through Cell-Cycle–Independent Regulation of E2f3a
Figure 3
Differentiation Defects in Rb KO SACs
(A) P18 horizontal sections of WT, Rb KO, and Rb/E2f1 DKO retina were stained for nuclei (DAPI, blue), Calb2 (green), and Slc18a3 (red).
(B) Confocal images of P30 horizontal sections of WT and Rb KO retina were stained for nuclei (DAPI, blue), Chat and Slc18a3 (both red), and Camk2a (green). In the Rb KO section, the red stain is Chat only, as Slc18a3 is missing (see [A]).
(C) Quantification of dense Calb2+ cell bodies in the INL, total Slc18a3+ cell bodies, and Camk2a+ cell bodies in the INL. Error bars represent SD of measurements from three animals, and asterisks indicate significant differences between retinas of WT and the indicated genotypes (*, p <0.05; **; p <0.01; ANOVA and Tukey HSD test).
Scale bars are 50 μm in (A) and 20 μm in (B).