Rb-Mediated Neuronal Differentiation through Cell-Cycle–Independent Regulation of E2f3a
Figure 2
E2f1 Deletion Rescues Ganglion, Rod, and Bipolar Cells in the Rb KO Retina
(A) Horizontal retinal sections from mice of the indicated ages and genotypes were stained for nuclei (DAPI, blue) and markers that detect ganglion cells (Pou4f2, red), rods and cones (Sag [rod arrestin], green), and rod bipolar cells (Prkca, green, and Cabp5, red). Scale bars are 50 μm.
(B) Quantification of Pou4f2+ ganglion cells.
(C) Quantification of Prkca+ and Cabp5+ bipolar cells.
(D) Thickness of the ONL, which represents the number of rods.
Error bars represent SD of measurements from three animals, and asterisks indicate a significant difference between retinas of WT and the indicated genotypes, unless indicated otherwise by connecting lines (*, p < 0.05; **; p < 0.01; ANOVA and Tukey HSD test).
(E and F) ERGs were recorded from the indicated genotypes under dark-adapted (scotopic) conditions, and (E) intensity series and (F) b-wave amplitudes as a function of the logarithm of the flash intensity were determined. (F) Further illustrates that the relative influence of the mutations on the photoreceptors (indicated by the saturated a-wave amplitude, right graph) was not substantially different from their effect on the b-wave response (dominated by the bipolars, left graph) at the same intensity of 10 cd·s/m2.