Structural and Thermodynamic Properties of Selective Ion Binding in a K+ Channel
Figure 5
ITC Titration of Alkaline Earth Metal Cations Binding to KcsA
(A) A BaCl2 solution is titrated into a solution containing KcsA.
(B) The total heat exchanged is fit to a single binding isotherm with KD and ΔH° as independent variables.
(C) A CaCl2 solution titrated into a solution containing KcsA shows no ion binding.