Assembly Factor Omp85 Recognizes Its Outer Membrane Protein Substrates by a Species-Specific C-Terminal Motif

doi:10.1371/journal.pbio.0040377

Assembly Factor Omp85 Recognizes Its Outer Membrane Protein Substrates by a Species-Specific C-Terminal Motif

Figure 2

E. coli Omp85 Forms Multimers

(A) Size exclusion chromatography of purified refolded Omp85. Symbols indicate elution volumes of the MW standard proteins thyroglobulin (669 KDa), ferritine (440 KDa), catalase (232 KDa), aldolase (158 KDa), and ovalbumin (67 KDa) (Amersham Bioscience).

(B) Blue Native PAGE gel of purified Omp85. The positions of molecular mass marker proteins are shown at the left (in KDa).

doi: https://doi.org/10.1371/journal.pbio.0040377.g002