Tomosyn Inhibits Synaptic Vesicle Priming in Caenorhabditis elegans
Figure 5
Comparison of the Plasma Membrane-Contacting Vesicles and Synaptic Vesicle Distribution in Cholinergic and GABAergic Synapses of WT, tom-1(nu468), and TOM-1A–Expressing Animals ( jaIs1052)
(A) Representative images of cholinergic synapses in tom-1(nu468) (top) and jaIs52 (bottom), Scale bar = 200 nm.
(B) The ratio of the plasma membrane-contacting vesicles per profile is reduced in tom-1(nu468) mutants expressing TOM-1A in cholinergic neurons ( jaIs1052) compared to both tom-1(nu468) mutants and WT.
(C) The distribution of plasma membrane-contacting vesicles relative to the presynaptic density in cholinergic synapses of jaIs1052 and tom-1(nu468) animals, expressed in 30-nm bins as ratio of total vesicles per profile.
(D) Representative images of a GABAergic synapse in tom-1(nu468) mutants (top) and jaIs1052 (bottom).
(E) The ratio of plasma membrane-contacting vesicles is significantly increased in tom-1(nu468) GABAergic synapses compared to WT, and is not rescued by expressing TOM-1A in cholinergic neurons.
(F) The distribution of plasma membrane-contacting vesicles relative to the PD are similar in tom-1(nu468) and jaIs1052 animals at GABAergic neuromuscular synapses. Data expressed as mean ± SEM.