Ankyrin-B Coordinates the Na/K ATPase, Na/Ca Exchanger, and InsP3 Receptor in a Cardiac T-Tubule/SR Microdomain
Figure 6
Reconstitution of the NCX1, NKA, and InsP3R Complex Requires Ankyrin-B
(A) Purified NKA (α/β subunits), InsP3R, NCX1, and ankyrin-B membrane-binding domain (ANKB MBD) were examined by SDS-PAGE and Coomassie blue.
(B) Stained gel of control beads and beads plus purified InsP3R.
(C) NKA and NCX1 were 125I-labeled and incubated with control Dynabeads or Dynabeads coated with InsP3R with or without ankyrin-B membrane-binding domain. Bound protein was analyzed by a gamma-counter (C) and by SDS-PAGE and phosphorimaging (not shown; see Materials and Methods for protein measurement, n = 3, p < 0.05).