Functional Analysis of Human Hematopoietic Stem Cell Gene Expression Using Zebrafish
Figure 2
Functional Genomics Screen in Zebrafish
The hematopoietic function of differentially expressed candidate genes was determined by injecting MOs into one- to two-cell embryos from gata1:DsRed Tg zebrafish to disrupt gene expression. Injected embryos were scored at 30–48 hpf for the presence of DsRed+ blood cells by fluorescence microscopy. Subsequently, MO-targeted embryos with gross hematopoietic defects were analyzed for the expression of the early hematopoietic markers gata1 and scl by whole-mount in situ hybridization, and the late hematopoietic markers hbae1 and lcp1 by Q-RT-PCR.