Extensive Association of Functionally and Cytotopically Related mRNAs with Puf Family RNA-Binding Proteins in Yeast
Figure 2
Strategy for Analyzing Genome-Wide RNA–Protein Interactions
Protein A-tagged Puf proteins were captured with IgG–Sepharose and released from the beads by cleavage with TEV protease. RNAs associated with the released proteins were isolated, and cDNA copies were fluorescently labeled and hybridized to yeast DNA microarrays. The Cy5/Cy3 fluorescence ratio for each locus reflects its enrichment by affinity for the cognate protein.