Effects of Gypsophila oldhamiana gypsogenin on growth and apoptosis of human colon cancer HT-29 cellsChinese Full Text
TIAN Gui-hong;QIN Ying;ZHOU Ling;ZHONG Ying;MU Yan-ling;Institute of Materia Medica,Shandong Academy of Medical Sciences;Department of Human Anatomy,Shandong Medical College;
Abstract: Objective To investigate the effects of Gypsophila oldhamiana gypsogenin（ GOG） on induction of apoptosis in human colon cancer HT- 29 cells. Methods The growth and apoptosis of HT- 29 cells treated with 5,10,20,40 μmol·L- 1GOG are investigated by MTT assay and flow cytometry. The mRNA expression of Caspase- 3 and Bax was measured by real- time fluorescence quantitative RT- PCR. Results GOG inhibited the proliferation of HT- 29 cell line in a dose- and time- dependent manner. Cell apoptosis rate increase obviously.Caspase- 3 and Bax mRNA expression quantity increase gradually,assumes the obvious density dependence. Conclusion GOG could inhibit HT- 29 cell proliferation through inducing cell apoptosis in vitro.The mechanism might be that GOG could enhance the level of Bax and activate the pathway of Caspase- 3.
Keywords：
colon cancer; Gypsophila oldhamiana gypsogenin; cell apoptosis; caspase-3; Bcl-2 associated X protein;
- DOI：
10.13699/j.cnki.1001-6821.2014.11.013
- Series：
- Subject：
- Classification Code：
R735.35
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