Condition of 3-phenylboronic acid degradation enzyme produced by Aspergillus oryzae and character of the enzymeChinese Full Text
YU Zhi-qiang;CHI Yuan-long;GAO Han;YAO Kai;College of Light Industry and Food, Sichuan University;College of Chemistry, Sichuan University;
Abstract: 3-phenoxybenzoic acid（3-PBA） is a common degradation intermediate product of pyrethroid insecticides. In this study, the strain Aspergillus oryzae M-4 with high tolerance and good degradation ability to 3-PBA was used. The effects of different incubation conditions on the activity of 3-PBA degradation enzyme produced by the strain M-4 were investigated. And properties of the 3-PBA degradation enzyme under different pH and temperature conditions were studied. It was found that the maximum activity of 3-PBA degradation enzyme was obtained as 53.42 U when the initial pH of medium, 3-PBA concentration, inoculation volume, incubation temperature and time were 6.5, 150 μg/g, 2.25 mL（spore suspension concentration was 107 cfu/mL）, 30 ℃ and 120 h, respectively. The optimum pH and temperature of the enzyme were 7.0 and 30 ℃, respectively, and the pH and temperature range for the enzyme with good stability were 5.5～7.0 and 5～30 ℃, respectively.
Keywords：
3-phenoxybenzoic acid; degradation enzyme; Aspergillus oryzae; enzyme production condition; enzymatic property;
- DOI：
10.13684/j.cnki.spkj.2014.06.004
- Series：
- Subject：
- Classification Code：
TS201.3
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