Optimization of degradation condition for gibberellic acid by Enterococcus mundtiiChinese Full Text
WU Shuang 1,TANG Jie 1,CHI Yuan-long 1,JIA Dong-ying 1,HUANG Hao 2,YAO Kai 1*(1.College of Light Industry and Food,Sichuan University,Chengdu 610065;2.Normal College,Chengdu University,Chengdu 610106)
Abstract: Enterococcus mundtii with high tolerance and degradation ability to gibberellic acid（GA 3） was used as objective strain in this study.Effects of environmental factors on GA 3 degradation were studied,and the optimized degradation condition for GA 3 by Enterococcus mundtii was obtained by using Plackett-Burman design and response surface methodology.Furthermore,synergistic effects of different environmental factors on GA 3 degradation were investigated.It was indicated that environmental factors showed great influences on GA 3 degradation,and loaded liquid volume,GA 3 concentration and incubation temperature were the main influence factors.In addition,the degradation ratio of GA 3 by Enterococcus mundtii reached 73.19% under the optimized degradation condition,increased by 17.61% than before.The built response surface model can fit well with degradation effects of GA 3 by Enterococcus mundtii under different environmental conditions.
- DOI：
10.13684/j.cnki.spkj.2013.02.035
- Series：
- Subject：
- Classification Code：
TS201.3
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