Optimization on two-step enzymatic hydrolysis of Ovalipes punctatus waste by response surface methodologyChinese Full Text
LI Jing,WANG Xiao-bin,CHEN Xiao-e *,FANG Xu-bo,YU Hui(1.Food and Pharmacy College,Zhejiang Ocean University,Zhoushan 316004;2.Joint Key Laboratory of Zhejiang Province for the Research of Fishery Processing Technology,Hangzhou 310018)
Abstract: Aimed at improving the utilization of crab,studied the hydrolysis of crab waste by trypsase and flavourzyme.On the basis of one-factor-at-a-time experiments,the effect of hydrolysis tempersture,pH,enzyme dosage and hydrolysis time on hydrolysis degree was explored to optimize the hydrolysis conditions of crab protein through Box-Benhnken response surface analysis on the basis of single-factor experiments.The optimal hydrolysis temperature,pH,enzyme dosage and hydrolysis time were found to be 50 ℃,7.5,1200 U/g,4 h,add trypsase fist,then add flavourzyme after 1.2 h,compound ratio of 2:1,water-to-material ratio of 3:1,Under these optimal hydrolysis conditions,the pre-dicted hydrolysis deress was 26.22%,which was consistent with the average of three replicates of 26.01% obtained in the validation experiments.
- DOI：
10.13684/j.cnki.spkj.2012.11.059
- Series：
- Subject：
- Classification Code：
TS254.9
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