Determination of Kaempferol in Camellia petelotii by HPLCChinese Full Text
QIN Lan-fang1,LAI Mao-xiang1,2,LIANG Bing1,QU Xin-cheng1,HUANG Yun-feng1,HU Qi-ming1(1.Guangxi Institute of Chinese Medicine and Pharmaceutical Sciences,Nanning,Guangxi,530022,China;2.Guangxi Key Laboratory of Traditional China Medicine Quality Standards,Nanning,Guangxi,530022,China)
Abstract: A HPLC method was established to determine kaempferol in Camellia petelotii（Merrll） Sealy.Ultimate（XB-C18）column was used as chromatographic column;The mobile phase was methanol-0.4%phosphoric acid solution（55∶45）;The flow rate was 1.0 ml·min-1 and the detector wavelength was set at 360 nm;The column temperature was maintained at room temperature.The calibration curve of kaempferol was in good linearity over the range of 0.1～0.5μg（r=0.9998）.The average recovery was 96.36%,RSD=3.07%（n=6）.This method is convenient,sensitive,reliable and suitable for determination of kaempferol in Camellia petelotii（Merrll） Sealy.
- DOI：
10.13656/j.cnki.gxkx.2012.04.006
- Series：
- Subject：
- Classification Code：
R29;O657.72
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