Abstract
A simple on-column preconcentration method for capillary electrophoretic determination of eight polyphenolic compounds (carnosic acid, cinnamic acid, caffeic acid and rosmarinic acid, quercetin, apigenin, luteolin and rutin) was devised. The method was applied for the assay of polyphenols in methanolic extract of the medicinal plant Orthosiphon Stamineus Benth. The analysis was carried out in fused silica capillaries (I.D. 50 μm, effective length 50 cm, total length 60 cm) with UV detection at 200 nm. The background electrolyte was 50 mM sodium tetraborate of pH 9.0 (adjusted with phosphoric acid). Large volume sample stacking with polarity switching was used for sensitivity enhancement. With sample injection representing 50% of capillary volume and polarity switching at 1.6 min, an average 90-fold enhancement of absorbance signal of the analytes was achieved. The calibration curves were linear (r = 0.9956–0.9994) in the range 0.2 to 1.8 μg mL−1 of an analyte. The repeatability of migration times and peak areas was characterized by RSD values 0.11–0.57 and 1.63–5.66%, respectively. The proposed method offers favourable limits of detection (9–16 ng mL−1) that compare well with those of LC.
Similar content being viewed by others
References
Tezuka Y, Stampoulis P, Banskota AH, Awale S, Tran KQ, Saiki I, Kadota S (2000) Chem Pharm Bull 48:1711–1719
Banskota AH, Tezuka Y, Le Tran Q, Kadota S (2003) Curr Top Med Chem 3:227–248
Loon YH, Wong HW, Yap SP, Yuen KH (2005) J Chromatogr B 816:161–166
Adam Y, Somchit MN, Sulaiman MR, Nasaruddin AA, Zuraini A, Bustamam AA, Zakaria ZA (2009) J Ethnopharmacol 124:154–158
Akanae W, Tsujihata M, Yoshioka I, Nonomura N, Okuyama A (2010) Urol Res 38:89–96
Olah NK, Radu L, Mogosan C, Hanganu D, Gocan S (2003) J Pharm Biomed Anal 33:117–123
Akowuah GA, Zhari I, Norhayati I, Sadikun A, Khamsah SM (2004) Food Chem 87:559–566
Sumaryono W, Proksch P, Wray V, Witte L, Hartmann T (1991) Planta Med 57:176–180
Olah NK, Hanganu D, Oprean R, Mogosan C, Dubei N, Gocan S (2004) J Planar Chromatogr Mod TLC 17:18–21
Hossain MA, Ismail Z (2005) Indian J Chem Sect B 44:436–437
Osbourn DM, Weiss DJ, Lunte CE (2000) Electrophoresis 21:2768–2779
Urbanek M, Krivankova L, Bocek P (2003) Electrophoresis 24:466–485
Bonoli M, Montanucci M, Toschi TG, Lercker G (2003) J Chromatogr A 1011:163–172
Tomasbarberan FA (1995) Phytochem Anal 6:177–192
Jac P, Polasek M, Pospisilova M (2006) J Pharm Biomed Anal 40:805–814
Urbanek M, Pospisilova M, Polasek M (2002) Electrophoresis 23:1045–1052
Urbanek M, Blechtova L, Pospisilova M, Polasek M (2002) J Chromatogr A 958:261–271
Hamoudova R, Urbanek M, Pospisilova M, Polasek M (2004) J Chromatogr 1032:281–287
Persat A, Chambers RD, Santiago JG (2009) Lab Chip 9:2437–2453
Breadmore MC, Thabano JRE, Dawod M, Kazarian AA, Quirino JP, Guijt RM (2009) Electrophoresis 30:230–248
Sun Y, Guo T, Sui Y, Li FM (2003) J Sep Sci 26:1203–1206
Baskan S, Oztekin N, Erim FB (2007) Food Chem 101:1748–1752
Pancorbo AC, Carretero AS, Gutierrez AF (2005) J Sep Sci 28:925–934
Petr J, Vitkova K, Ranc V, Znaleziona J, Maier V, Knob R, Sevcik J (2008) J Agric Food Chem 56:3940–3944
Safra J, Pospisilova M, Kavalirova A (2006) J Pharm Biomed Anal 41:1022–1024
Acknowledgment
The financial support of the Czech Ministry of Education, Grant MSM 0021620822, and SVV-2010-261-001 is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Honegr, J., Šafra, J., Polášek, M. et al. Large-Volume Sample Stacking with Polarity Switching in CE for Determination of Natural Polyphenols in Plant Extracts. Chroma 72, 885–891 (2010). https://doi.org/10.1365/s10337-010-1752-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-010-1752-1