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Simultaneous Quantitative Analysis of Shikonin and Deoxyshikonin in Rat Plasma by Rapid LC–ESI–MS–MS

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Abstract

The purpose of this article was to develop a rapid and robust LC–MS–MS method for quantifying shikonin and deoxyshikonin simultaneously in rat plasma using emodin as internal standard. The LC system consisted of an Agilent ZORBAX SB-C18 (1.8 μm, 250 × 4.6 mm, 20 °C) column. Elution with an isocratic mobile phase consisted of methanol/10 mM ammonium acetate in water/acetonitrile containing 0.05% formic acid (45:10:45, v/v/v) at a flow rate of 0.8 mL min−1 yielded sharp, high-resolved peaks within 12 min. The lower limits of quantitation were 0.5 ng mL−1 for shikonin, and 8 ng mL−1 for deoxyshikonin. Correlation coefficient (r) values for the linear range of two analytes were greater than 0.99. Assay precision was <13% and accuracy was 87–99%. This newly developed method was used to the pharmacokinetic studies of the shikonin analogues in rats after intravenous administration (n = 4).

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Authors and Affiliations

  1. Department of Medical Oncology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China

    Sui Huang, Lanfang Yu & Peng Shen

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  1. Sui Huang
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  2. Lanfang Yu
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  3. Peng Shen
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Correspondence to Peng Shen.

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Huang, S., Yu, L. & Shen, P. Simultaneous Quantitative Analysis of Shikonin and Deoxyshikonin in Rat Plasma by Rapid LC–ESI–MS–MS. Chroma 72, 63–69 (2010). https://doi.org/10.1365/s10337-010-1599-5

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  • DOI: https://doi.org/10.1365/s10337-010-1599-5

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