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Molecularly Imprinted Monolithic Column for Selective On-Line Extraction of Enrofloxacin and Ciprofloxacin from Urine

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Abstract

A simple on-line molecularly imprinted solid-phase extraction coupled with liquid chromatographic separation was developed for the simultaneous determination of enrofloxacin and its active metabolite ciprofloxacin from urine samples. The molecularly imprinted monolithic columns were synthesized in water-containing systems using norflorxacin as dummy template and methanol–water (5:1, v/v) as porogenic solvent, which reveal high affinity to enrofloxacin and ciprofloxacin in the aqueous environment. Due to the unique porous structure and flow-through channels existing in the network skeleton of the imprinted monolith, urine samples could be injected directly into the imprinted column, proteins and other biological matrix were quickly washed out and the analytes were selectively retained and enriched. Good linearity was obtained from 0.05 to 200 mg L−1 with relative standard deviations less than 3.1%. The recoveries were higher than 87% at three different concentrations and the limit of detection of the method was 0.01 mg L−1. Moreover, by increasing the injection volume, the sensitivity of the method could be further improved.

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Acknowledgments

The authors gratefully appreciate the financial support by Inha University Research Grant (INHA-2009).

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Authors and Affiliations

  1. College of Pharmacy, Hebei University, 071002, Baoding, China

    Hongyuan Yan

  2. Department of Chemical Engineering, Inha University, Inchon, 402751, Korea

    Hongyuan Yan & Kyung Ho Row

  3. Department of Chemistry, Baoding University, 071000, Baoding, China

    Fengxia Qiao

Authors
  1. Hongyuan Yan
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  2. Fengxia Qiao
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  3. Kyung Ho Row
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Correspondence to Kyung Ho Row.

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Yan, H., Qiao, F. & Row, K.H. Molecularly Imprinted Monolithic Column for Selective On-Line Extraction of Enrofloxacin and Ciprofloxacin from Urine. Chroma 70, 1087–1093 (2009). https://doi.org/10.1365/s10337-009-1244-3

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  • DOI: https://doi.org/10.1365/s10337-009-1244-3

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