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LC Fingerprint and Hierarchical Cluster Analysis of Crocus sativus L. from Different Locations in China

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Abstract

Using simple, sensitive and specific column liquid chromatography, a chemical fingerprinting method was developed for investigating and demonstrating the variance of chemical components among different populations of Crocus sativus L., from 15 locations in China. The LC assay was performed on a reversed-phase C18 column with linear gradient elution using methanol and 1% aqueous acetic acid. The LC data showed considerable variation of chemical constituents among C. sativus populations. Four chemotypes were visually developed from the chromatographic profiles. The grouping of 15 C. sativus populations in hierarchical clustering analysis was in good agreement with the visual comparison of their chromatograms, as demonstrated by chemotypes. In addition, the bioactive compound crocin 1 in this herb was quantitatively determined by a validated reversed-phase LC analysis. Relative peak areas of characteristic compounds were also calculated for quantitative expression of the LC fingerprints. These results showed that the established method for fingerprinting analysis was considered to be suitable for the quality control of C. sativus.

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Acknowledgments

We are grateful to Mr. Wei-Zhong Pei for providing the analyzed saffron samples. Thanks are due to Miss Liu-Liu Wu for LC technical assistance. This work was supported by Shanghai Science Plan Foundation of China (No. 05DZ19729).

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Authors and Affiliations

  1. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, 200433, Shanghai, People’s Republic of China

    Yang Wang, Ting Han, Xiao-Gang Zhang, Cheng-Jian Zheng & Lu-Ping Qin

  2. Faculty of Science, School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, Liverpool, L33AF, UK

    Khalid Rahman

Authors
  1. Yang Wang
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  2. Ting Han
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  3. Xiao-Gang Zhang
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  4. Cheng-Jian Zheng
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  6. Lu-Ping Qin
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Corresponding author

Correspondence to Lu-Ping Qin.

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Y. Wang and T. Han have contributed equally to this manuscript.

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Wang, Y., Han, T., Zhang, XG. et al. LC Fingerprint and Hierarchical Cluster Analysis of Crocus sativus L. from Different Locations in China. Chroma 70, 143–149 (2009). https://doi.org/10.1365/s10337-009-1103-2

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  • DOI: https://doi.org/10.1365/s10337-009-1103-2

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