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A Validated RP-LC Method for the Determination of Isosorbide 5-Mononitrate in a Leishmanicidal Ointment

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Abstract

An RP-LC method using a YMC-Pack ODS-AQ column and UV detection (220 nm) was validated for the determination of isosorbide 5-mononitrate selected as an exogenous NO source in a leishmanicidal ointment. After extraction with hot water, the extracts were analysed at 35 °C using isocratic conditions (water-acetonitrile, 4:1 v/v; 0.9 mL min−1). The method was specific, precise, accurate and linear.

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Authors and Affiliations

  1. Laboratoire de Chimie Analytique, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076, Bordeaux cedex, France

    Philippe Dallet, Tina Kauss, Marie-Hélène Langlois & Jean-Pierre Dubost

  2. Laboratoire de Pharmacie Galénique, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076, Bordeaux cedex, France

    Fawaz Fawaz

  3. Laboratoire d’Immunologie et de Parasitologie, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076, Bordeaux cedex, France

    Djavad M. Mossalayi

Authors
  1. Philippe Dallet
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  2. Tina Kauss
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  3. Marie-Hélène Langlois
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  4. Fawaz Fawaz
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  5. Djavad M. Mossalayi
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  6. Jean-Pierre Dubost
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Correspondence to Philippe Dallet.

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Dallet, P., Kauss, T., Langlois, MH. et al. A Validated RP-LC Method for the Determination of Isosorbide 5-Mononitrate in a Leishmanicidal Ointment. Chroma 69, 1129–1131 (2009). https://doi.org/10.1365/s10337-009-0998-y

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  • DOI: https://doi.org/10.1365/s10337-009-0998-y

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