Abstract
A simple, sensitive, and precise thin layer chromatographic (TLC) method for simultaneous analysis of psychopharmacological drugs like amitriptyline HCl, trifluoperazine HCl, risperidone and alprazolam in their single dosage forms has been developed, validated, and used for determination of the compounds in commercial pharmaceutical products. The TLC separation was carried out on Merck TLC aluminium sheets of silica gel 60 F254 using carbon tetrachloride:acetone:triethylamine (8:2:0.3, v/v/v), as mobile phase. Densitometric measurements of their spots were achieved at 250 nm over the concentration range for amitriptyline HCl (50–1,200 ng spot−1), trifluoperazine HCl (50–1,200 ng spot−1), risperidone (100–2,400 ng spot−1) and alprazolam (25–600 ng spot−1). Limit of detection (LOD) for amitriptyline HCl (20 ng spot−1), trifluoperazine HCl (20 ng spot−1), risperidone (40 ng spot−1) and alprazolam (5 ng spot−1) was obtained. The study showed that TLC was sensitive and selective for determination of amitriptyline HCl, trifluoperazine HCl, risperidone and alprazolam using a single mobile phase. This proposed method is able for simultaneous determination of psychopharmacological drugs and also applicable for analysis of pharmaceutical formulations.
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El-Gendy AE, El-Bardicyy MG, Loutfy HM, El-Tarras MF (1993) Spectro Lett 26:1649–1660
Markopoulou CK, Malliou ET, Koundourellis JE (2005) J Pharm Biomed Anal 37:249–258
Smith GA, Schulz P, Giacomini KM, Blaschke TF (1981) J Pharm Sci 71:581–583
Deshmane GV, Vakil JR, Dhaneshwar SR, Mahadik KR, Kadam SS (1997) Indian Drugs 34:443–445
El-Gindy A, El-Zeany B, Awad T, Marwan MS (2001) J Pharm Biomed Anal 26:203–210
Ruedas Rama MJ, Ruiz Medina A, Molina Diaz A (2004) J Pharm Biomed Anal 35:1027–1034
El-Gindy A, El-Zeany B, Awad T, Marwan MS (2002) J Pharm Biomed Anal 27:9–18
Pradas TN, Sivakumar M (1992) Pharmazie 47:231
Basavaiah K, Gowda PN, Prameela HC, Somashekhar BC (2005) Indian Drugs 42:585–591
Schatz DS, Saria A (2000) Int J Exp Clin Pharmacol 60:51–56
Raggi MA, Bugamelli F, Sabbioni C, Addolorata SM, Petio C (2005) J Sep Sci 28:245–250
Verma JK, Joshi AV (2005) Indian Drugs 42:805
Nudelman NS, Cabrera CG (2002) J Pharm Biomed Anal 30:887–893
Yamuna S, Vaidhyalingam V (2004) Indian Drugs 41:413
Nagasaki T, Ohkubo T, Sugawara K, Yasui N, Otani K, Kaneko S (1997) Anal Sci 13:245–249
Crouch DJ, Rollins DE, Canfield DV, Andrenyak DM, Schulties JE (1999) J Anal Toxicol 23:479–485
Kronstrand R, Nystrom I, Josefsson M, Hodgins S (2002) J Anal Toxicol 26(7):479–484
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Patel, S.K., Patel, N.J. TLC Determination of Amitriptyline HCl, Trifluoperazine HCl, Risperidone and Alprazolam in Pharmaceutical Products. Chroma 69, 393–396 (2009). https://doi.org/10.1365/s10337-008-0870-5
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DOI: https://doi.org/10.1365/s10337-008-0870-5