Skip to main content
SpringerLink
Log in

Optimized Conditions of Enantioseparation of β-Blockers by CZE Using Carboxymethyl-β-Cyclodextrin as Chiral Selector

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Capillary zone electrophoresis was used for the enantiomeric separation of six β-blocking drug substances with β-cyclodextrin (β-CD) and its derivatives as chiral selectors employing an uncoated capillary. The effects of pH value and composition of the background electrolyte (BGE), the capillary temperature and running voltage have been investigated. The results showed that β-CD type, concentration and pH value have a strong influence on the efficiency of the chiral separation. Carboxymethyl-β-cyclodextrin (CM-β-CD) gave a baseline enantiomeric separation for six β-blocking drug substances under optimal conditions, whereas the β-CD, hydroxypropyl-β-cyclodextrin (HP-β-CD) showed no chiral recognition. The potential and capillary temperature did not have a great effect on enantiomer resolution.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Nagata Y, Iida T, Sakai M (2001) J Mol Catal B Enzym 12:105–108. doi:10.1016/S1381-1177(00)00209-5

    Article  CAS  Google Scholar 

  2. Myrdal PB, Angersbach BS, Karlage K, Kuehl PJ (2006) J Chromatogr A 1132:315–319. doi:10.1016/j.chroma.2006.07.010

    Article  CAS  Google Scholar 

  3. Guo ZS, Wang H, Zhang YS (2006) J Pharm Biomed Anal 41:310–314. doi:10.1016/j.jpba.2005.10.045

    Article  CAS  Google Scholar 

  4. Stringham RW, Ye YK (2006) J Chromatogr A 1101:86–93. doi:10.1016/j.chroma.2005.09.065

    Article  CAS  Google Scholar 

  5. Paik MJ, Nguyen DT, Kim KR (2006) J Chromatogr A 1103:177–181. doi:10.1016/j.chroma.2005.11.078

    Article  CAS  Google Scholar 

  6. Liu Y, Berthod A, Mitchell CR, Xiao TL, Zhang B, Armstrong DW (2002) J Chromatogr A 978:185–204. doi:10.1016/S0021-9673(02)01356-0

    Article  CAS  Google Scholar 

  7. Zukowski J, Biasi VD, Berthod A (2002) J Chromatogr A 948:331–342. doi:10.1016/S0021-9673(01)01473-X

    Article  CAS  Google Scholar 

  8. Li GB, Lin XL, Zhu CF, Hao AY, Guan YF (2000) Anal Chim Acta 421:27–34. doi:10.1016/S0003-2670(00)00960-0

    Article  CAS  Google Scholar 

  9. Maier V, Horáková J, Petr J, Tesařová E, Coufal P, Ševčík J (2005) J Pharm Biomed Anal 39:691–696. doi:10.1016/j.jpba.2005.04.023

    Article  CAS  Google Scholar 

  10. Vargas MG, Heyden YV, Maftouh M, Massart DL (1999) J Chromatogr A 855:681–693. doi:10.1016/S0021-9673(99)00701-3

    Article  CAS  Google Scholar 

  11. Ceccato A, Huert P, Crommen J (1997) J Chromatogr A 760:193–203. doi:10.1016/S0021-9673(96)00784-4

    Article  CAS  Google Scholar 

  12. Petersen PV, Ekelund J, Olsen L, Ovesen SV (1997) J Chromatogr A 757:65–71. doi:10.1016/S0021-9673(96)00677-2

    Article  Google Scholar 

  13. Agrawal YK, Patel RN (2005) J Chromatogr B Analyt Technol Biomed Life Sci 820:23–31. doi:10.1016/j.jchromb.2005.03.003

    Article  CAS  Google Scholar 

  14. Elena B, Acquarica ID, Gasparrini F, Lalli S, Misiti D, Pazzucconi F et al (2003) J Chromatogr B Analyt Technol Biomed Life Sci 796:45–54. doi:10.1016/j.jchromb.2003.07.001

    Article  Google Scholar 

  15. Zhang XH, Yang JO, Baeyens WRG, Zhai SD, Yang YP, Huang GM (2003) J Pharm Biomed Anal 31:1047–1057. doi:10.1016/S0731-7085(02)00697-0

    Article  CAS  Google Scholar 

  16. Dakers JM, Boulton DW, Fawcett JP (1997) J Chromatogr B Analyt Technol Biomed Life Sci 704:215–220. doi:10.1016/S0378-4347(97)00422-2

    Article  CAS  Google Scholar 

  17. Martinez-Pla JJ, Martin-Biosca Y, Sagrado S, Villanueva-Camañas RM, Medina-Hernández MJ (2004) J Chromatogr A 1048:111–118

    CAS  Google Scholar 

  18. Kim E, Koo YM, Chunga DS (2004) J Chromatogr A 1045:119–124. doi:10.1016/j.chroma.2004.06.006

    Article  CAS  Google Scholar 

  19. Hui F, Ekborg-Ott KH, Armstrong DW (2001) J Chromatogr A 906:91–103. doi:10.1016/S0021-9673(00)00954-7

    Article  CAS  Google Scholar 

  20. Vescina MC, Fermier AM, Guo Y (2002) J Chromatogr A 973:187–196. doi:10.1016/S0021-9673(02)01192-5

    Article  CAS  Google Scholar 

  21. Zhou SS, Yang JO, Baeyens WRG, Zhao HC, Yang YP (2006) J Chromatogr A 1130:296–301. doi:10.1016/j.chroma.2006.05.055

    Article  CAS  Google Scholar 

  22. Eeckhaut AV, Detaevernier MR, Michotte Y (2004) J Pharm Biomed Anal 36:799–805. doi:10.1016/j.jpba.2004.08.024

    Article  Google Scholar 

  23. Wang H, Gu JL, Hu HF, Dai RJ, Ding TH, Fu RN (1998) Anal Chim Acta 359:39–46. doi:10.1016/S0003-2670(97)00655-7

    Article  CAS  Google Scholar 

  24. Lim HK, Linh PT, Hong CH, Kim KH, Kang JS (2001) J Chromatogr B Analyt Technol Biomed Life Sci 755:259–264. doi:10.1016/S0378-4347(01)00118-9

    Article  CAS  Google Scholar 

  25. Pak C, Marriott PJ, Carpenter PD, Amiet RG (1998) J Chromatogr A 793:357–364. doi:10.1016/S0021-9673(97)00919-9

    Article  CAS  Google Scholar 

  26. Goel TV, Nikelly JG, Simpson RC, Matuszewski BK (2004) J Chromatogr A 1027:213–221. doi:10.1016/j.chroma.2003.08.082

    Article  CAS  Google Scholar 

  27. Li F, Cooper SF, Mikkelsen SR (1995) J Chromatogr B Analyt Technol Biomed Life Sci 674:277–283. doi:10.1016/0378-4347(95)00307-X

    Article  CAS  Google Scholar 

  28. Martínez-Pla JJ, Martín-Biosca Y, Sagrado S, Villanueva-Camañas RM, Medina-Hernández MJ (2004) Anal Chim Acta 507:175–182. doi:10.1016/j.aca.2003.11.029

    Article  Google Scholar 

  29. Perrin C, Vander Heyden Y, Maftouh M, Massart DL (2001) Electrophoresis 22:3202–3215. doi :10.1002/1522-2683(200109)22:15<3203::AID-ELPS3203>3.0.CO;2-G

    Article  Google Scholar 

  30. Fillet M, Hubert P, Crommen J (1998) Electrophoresis 19:2834–2840. doi:10.1002/elps.1150191608

    Article  CAS  Google Scholar 

  31. Marini RD, Servais AC, Rozet E, Chiap P, Boulanger B, Rudaz S, Crommen J, Hubert P, Fillet M (2006) J Chromatogr A 1120:102–111. doi:10.1016/j.chroma.2006.03.104

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, Zhengzhou University, 450001, Zhengzhou, Henan, People’s Republic of China

    Hongling Zhang, Hong Shao, Youmei A & Zhenzhong Zhang

  2. Research Center of Nanotechnology for Drug, Zhengzhou University, 450001, Zhengzhou, Henan, People’s Republic of China

    Hongling Zhang, Hong Shao, Youmei A & Zhenzhong Zhang

  3. Henan Key Laboratory for Molecular Medicine, 450052, Zhengzhou, Henan, People’s Republic of China

    Hongling Zhang & Hong Shao

Authors
  1. Hongling Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hong Shao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Youmei A
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhenzhong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhenzhong Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, H., Shao, H., A, Y. et al. Optimized Conditions of Enantioseparation of β-Blockers by CZE Using Carboxymethyl-β-Cyclodextrin as Chiral Selector. Chroma 68, 653–658 (2008). https://doi.org/10.1365/s10337-008-0747-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-008-0747-7

Keywords

Search

Navigation