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Norchlorotestosterone Acetate: An Alternative Metabolism Study and GC–MS2 Analysis in Kidney Fat, Urine, and Faeces

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Abstract

Norchlorotestosterone acetate (NClTA) is an anabolic steroid which resembles chlorotestosterone acetate. It cannot yet be detected by routine methods used for anabolic steroids, because there is no knowledge of its metabolic pathway. The invertebrate Neomysis integer has been used as an alternative model to study the metabolism of NClTA. The experimental results indicated the presence of 4-norchloroandrost-4-ene-17-ol-3-one (NClT) and 4-norchloroandrost-4-ene-3,17-dione (NorClAD) as possible metabolites of NClTA. Subsequently NClTA and the synthesised metabolites NClT and NorClAD were incorporated into the routine multi-residue method for detection of anabolic steroids in kidney fat, urine, and faeces.

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Authors and Affiliations

  1. Ghent University, Department of Veterinary Public Health and Food Safety, Laboratory of Chemical Analysis, Salisburylaan, 133 9820, Merelbeke, Belgium

    N. Van Hoof, K. De Wasch, S. Poelmans, H. Noppe & H. De Brab

  2. Catholic Technical University, St-LievenDepartment of Industrial Engineer, Laboratory of Chemistry, Gebroeders Desmetstraat, 1 9000, Gent, Belgium

    D. Bruneel & S. Spruyt

  3. Ghent University, Laboratory of Environmental Toxicology and Aquatic Ecology, J. Plateaustraat, 22 9000, Gent, Belgium

    C. Janssen

  4. Federal Food Laboratory Braemkasteelstraat, 59 9050, Gentbrugge, Belgium

    D. Courtheyn

Authors
  1. N. Van Hoof
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  2. K. De Wasch
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  3. S. Poelmans
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  4. D. Bruneel
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  5. S. Spruyt
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  6. H. Noppe
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  7. C. Janssen
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  8. D. Courtheyn
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  9. H. De Brab
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Corresponding author

Correspondence to N. Van Hoof.

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In Honour of Professor Edward Houghton: A Celebration of 30 Years in Racing Chemistry

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Van Hoof, N., De Wasch, K., Poelmans, S. et al. Norchlorotestosterone Acetate: An Alternative Metabolism Study and GC–MS2 Analysis in Kidney Fat, Urine, and Faeces. Chromatographia 59 (Suppl 1), S85–S93 (2004). https://doi.org/10.1365/s10337-003-0178-4

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  • DOI: https://doi.org/10.1365/s10337-003-0178-4

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