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Optimization of Micellar LC Conditions for the Flavonoid Separation

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Abstract

The chromatographic conditions for the separation of a complex set of flavonoids (aglycones and glycosides) by micellar liquid chromatography with spectrophotometric detection were optimized. A good separation for all analytes was obtained and satisfactory peak shapes were achieved by isocratic elution with Ultrasphere ODS column (250 mm × 4.6 mm, 5 μm). The optimal mobile phase range for flavonoids separation is: SDS concentration between 0.014 and 0.018 mol L−1 and 1-propanol volume fraction between 2.2 and 4.5% (v/v) in a diluted (1:5) phosphate buffer solution pH 6.86. The flavonoids (robinin, rutin, hyperoside, quercitrin, liquroside, luteolin-7O-glucoside, apigenin-7O-glucoside, isosalipurposide, myricetin, fisetin, luteolin, apigenin, quercetin and caempferol) were successfully separated within 40 min with isocratic elution. The developed method is an alternative to reversed-phase LC in the assay of flavonoids in plants, plant extracts and plant extract containing drugs.

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Authors and Affiliations

  1. Laboratory of Pharmacopoeial Analysis, Scientific and Expert Pharmacopoeial Centre, Astronomicheskaya Street 33, 61085, Kharkov, Ukraine

    Artem U. Kulikov

  2. Department of Chemical Metrology, Kharkov V.N. Karazin National University, Svoboda Square 4, 61077, Kharkov, Ukraine

    Artem U. Kulikov, Marina N. Galat & Alexander P. Boichenko

Authors
  1. Artem U. Kulikov
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  2. Marina N. Galat
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  3. Alexander P. Boichenko
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Correspondence to Artem U. Kulikov.

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Kulikov, A.U., Galat, M.N. & Boichenko, A.P. Optimization of Micellar LC Conditions for the Flavonoid Separation. Chroma 70, 371–379 (2009). https://doi.org/10.1365/s10337-009-1164-2

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