Background Reduction with Two-Color Two-Beam Multiphoton Excitation

Demirhan Kobat; Guanghao Zhu; Chris Xu

doi:10.1364/BIOMED.2008.BMF6

Biomedical Optics
OSA Technical Digest (CD) (Optica Publishing Group, 2008),
paper BMF6
•https://doi.org/10.1364/BIOMED.2008.BMF6

Background Reduction with Two-Color Two-Beam Multiphoton Excitation

Demirhan Kobat, Guanghao Zhu, and Chris Xu

Biomedical Optics 2008

St. Petersburg, Florida United States
16–19 March 2008
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Advances in Microscopy (BMF)

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D. Kobat, G. Zhu, and C. Xu, "Background Reduction with Two-Color Two-Beam Multiphoton Excitation," in Biomedical Optics, OSA Technical Digest (CD) (Optica Publishing Group, 2008), paper BMF6.

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Abstract

By comparing axial excitation profiles, we present experimental proof which demonstrates that two-color two-beam multiphoton excitation offers a better signal to background ratio than conventional single beam multiphoton excitation.

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