Abstract

A holographic microscopy reconstruction method compatible with a high numerical aperture microscope objective (MO) up to $\mathrm{NA}=1.4$ is proposed. After off-axis and reference field curvature corrections, and after selection of the $+1$ grating order holographic image, a phase mask that transforms the optical elements of the holographic setup into an afocal device is applied in the camera plane. The reconstruction is then made by the angular spectrum method. The field is first propagated in the image half-space from the camera to the afocal image of the MO optimal plane (the plane for which the MO has been designed) by using a quadratic kernel. The field is then propagated from the MO optimal plane to the object with the exact kernel. Calibration of the reconstruction is made by imaging a calibrated object such as a USAF resolution target for different positions along $z$. Once the calibration is done, the reconstruction can be made with an object located in any plane $z$. The reconstruction method has been validated experimentally with a USAF target imaged with a $\mathrm{NA}=1.4$ microscope objective. Near-optimal resolution is obtained over an extended range ($\pm 50\mathrm{\mu m}$) of $z$ locations.

© 2015 Optical Society of America

Holographic microscopy reconstruction in both object and image half-spaces with an undistorted three-dimensional grid

Nicolas Verrier, Danier Alexandre, Gilles Tessier, and Michel Gross
Appl. Opt. 54(15) 4672-4677 (2015)

Fast numerical reconstruction technique for high-resolution hybrid holographic microscopy

Yasuhiro Takaki and Hitoshi Ohzu
Appl. Opt. 38(11) 2204-2211 (1999)

Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms

Etienne Cuche, Pierre Marquet, and Christian Depeursinge
Appl. Opt. 38(34) 6994-7001 (1999)

High resolution digital holographic synthetic aperture applied to deformation measurement and extended depth of field method

Daniel Claus
Appl. Opt. 49(16) 3187-3198 (2010)

Extension of refocus depth range in digital holographic microscopy

Ming Tang, Zhi Yuan Tang, Xin Qiao, Kai Qi Lang, Yi Sun, and Xiao Ping Wang
Appl. Opt. 59(28) 8540-8552 (2020)